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KMID : 0043320040270111141
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Archives of Pharmacal Research
2004 Volume.27 No. 11 p.1141 ~ p.1146
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Inhibition of Trypsin-Induced Mast Cell Activation by Water Fraction of Lonicera japonica
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Kang Ok-Hwa
Choi Yeon-A
Park Hye-Jung
Lee Joo-Young
Kim Dae-Ki
Choi Suck-Chei
Kim Tae-Hyeon
Nah Yong-Ho
Yun Ki-Jung
Choi Suck-Jun
Kim Young-Ho
Bae Ki-Hwan
Lee Young-Mi
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Abstract
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Lonicera japonica Thunb.(Caprifoliaceae) has long been known as an anti-inflammatory. In the present study, the effect of water fraction of Lonicera japonica (LJ) on trypsin-induced mast cell activation was examined. HMC-1 cells were stimulated with trypsin (100 nM) in the presence or absence of LJ (10, 100, and 1000 g/mL). TNF- and tryptase production were measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-PCR. Extracellular signal-regulated kinase (ERK) phosphorylation was assessed by Western blot. Trypsin activity was measured by using Bz-DL-Arg-p-nitroanilide (BAPNA) as substrate. LJ (10, 100, and 1000 g/mL) inhibited TNF- secretion in a dose-dependent manner. LJ (10, 100, and 1000 g/mL) also inhibited TNF- and tryptase mRNA expression in trypsin-stimulated HMC-1. Furthermore, LJ inhibited trypsin-induced ERK phosphorylation. However, LJ did not affect the trypsin activity even 1000 g/mL. These results indicate that LJ may inhibit trypsin-induced mast cell activation through the inhibition of ERK phosphorylation than the inhibition of trypsin activity.
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KEYWORD
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Lonicera japonica, Trypsin, Tryptase, TNF-α, ERK
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